CHE 4246 - Biochemistry II Laboratory

Credits: 2 hrs

This course will focus on the theory and practice of experimental biochemistry and molecular biology. Laboratory techniques and concepts will include spectroscopic analysis of protein and nuclei acids, isolation and purification of biomolecules by chromatography (gel filtration, ion exchange and reversed phase), and analysis by gel electrophoresis. Experiments will also include techniques in protein crystallization, enzyme kinetics study, and evaluation of protein structure and stability by Circular Dichroism and Fluorescence. Biochemical and molecular biology techniques for the expression of a protein in bacteria will include transformation, screening, and propagation, isolation of the expressed protein and its purification and characterization. (Two three-hour laboratory periods per week.) Prerequisite(s): CHE 3146 and BIO 3431. Corequisite(s): CHE 4346.